Klinik und Poliklinik für Hals-, Nasen- u. Ohrenkranke
Josef-Schneider-Str. 11, 97080 Würzburg
Research foci (and basic equipment-based research projects):
General anesthesia in new born rodents for experimental microsurgery of the ear
Experimental investigations on laboratory animals usually require sufficient anesthesia with adequate analgesia and sedation. The technique used should be reliable and easily controllable by the investigator. Here, we present a technique for anesthesia to facilitate invasive and non-invasive investigations in new born rats and mice. Using a custom made breathing mask, anesthesia was induced in these animals with inhalation of gaseous nitrous oxide-oxygen (equal volume at 1 l/min) and halothane (3 vol.%). To maintain anesthesia, halothane insufflation was reduced to 1-1,5 vol.%. After completion of the experimental procedure, the application of the inhalative gases was determined and substituted by oxygen at 2 l/min. Anesthesia was performed in spontaneously breathing animals. Heart frequency and oxygenation were monitored using a commercially available pulse oximeter. Using the above described technique in neonatal rodents, microsurgery of the ear was performed without signs of pain or major bleeding. Auditory brain stem responses were recorded clearly and reproducable. The presented method represents a non-invasive, well tolerated and easy controllable anesthetical procedure which has proven to provide a sufficient and reliable sedation in neonatal rodents for surgical and non-surgical investigations.
Fully equipped operation room with anesthesia facilities for microsurgery in experimental animals.