Experimental studies on the influence of the local immune reaction on pain-associated behavior after nerve lesion: modification of the cytokine cascade
Project management at the University of Würzburg:
We investigated the link between cytokine inhibition, reduction of hyperalgesia and regeneration in painful neuropathies. Briefly, an early increase in TNF and a loss of IL-10 in the nerve were associated with the onset of pain related behavior in the animals. Blockade of TNF reduced hyperalgesia and blocked the reduction of IL-10. TNF-receptors, in paricular TNF-R2 were upregulated early and protractedly, proposing a potential therapeutic target. In the model of chronic constrictive nerve injury (CCI) used in our previous studies, the presence of a foreign body (ligatures) might be causal for the cytokine upregulation. We therefore established a new model, the ‚partial sciatic nerve transection' (PST) without the presence of a foreign body. This model leads to reproducable hyperalgesia, cytokine upregulation, and hyperalgesia can be reduced by cytokine blockade. Thus nerve lesion by itself and not the foreign body reaction are responsible for the pain associated behavior. Also, successful nerve regeneration was correlated with a decrease in hyperalgesia.
tumor necrosis factor-alpha
partial sciatic nerve transection
from 07.1998 to 07.2001
VW-Stiftung ,Granting date: 25.02.1998
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(2002). Epidermal innervation density after partial sciatic nerve lesion and pain related behavior in the rat. Acta Neuropathol, 2,1. (scientific article)