Third-party-funded project
Title:
Diagnostics of endothelial dysfunction
Project management at the University of Würzburg:
Participating scientists:
- Dr. med. Albert Smolenski
, Institut f. Biochemie II, Universitätsklinikum, 60590 Frankfurt/Main (ab 1.7.02),Tel: 069/6301-5569,Fax: 069/6301-5577
- Dr. rer. nat. Jörg Geiger
,Tel: 0931/201-45269,Fax: 0931/201-45153,Mail: geiger@klin-biochem.uni-wuerzburg.de
- Dr. rer. nat. Thomas Jarchau
,Tel: 0931/201-45136,Fax: 0931/201-45153,Mail: jarchau@klin-biochem.uni-wuerzburg.de
- Prof. Dr. med. Th. Münzel
, Univ.Krankenhaus Eppendorf, Abtl. Kardiologie, Martinistr. 52, 20246 Hamburg,Tel: 040/42803-3988,Fax: 040/42803-5862,Mail: muenzel@uke.uni-hamburg.de
- PD Dr. med. Martin Eigenthaler
,Tel: 0931/201-70080,Fax: 0931/201-45153,Mail: eigentha@klin-biochem.uni-wuerzburg.de
Abstract:
Aim of this project is the development of novel biochemical markers and functional paramaters for the human vascular endothelium. In particular, the analysis of VASP (vasodialtor-stimulated phosphoprotein) phosphorylation as indicator of the functional integrity of the endothelium is investigated. Three main question are addressed :
? The analysis of VASP phosphorylation as marker for endothelial functions in various animal model.
? The analysis of P-VASP as marker of endothelial functions in human vessel wall samples
? Developmemt of a P-VASP assay suitable for whole blood
This project is a collaboration with vasopharm Biotech GmbH (Wuerzburg)
Key words:
NO-cGMP Signalling
P-VASP
VASP
Endothelial Dysfunction
Projekt period:
from 12.2001 to 09.2002
Funding institution:
Wirtschaftsunternehmen ( Vasopharm Biotech ) ,Granting date: 19.12.2001
Publications:
- Geiger J, Brich J, Honig-Liedl P, Eigenthaler M, Schanzenbacher P, Herbert JM, Walter U..
(1999). Specific impairment of human platelet P2Y(AC) ADP receptor- mediated signalling by the antiplatelet drug clopidogrel.. Arteriosclerosis Thrombosis and Vascular Biology.;19:2007-2011, (scientific article)
- Schwarz UR, Geiger J, Walter U, Eigenthaler M..
(1999). Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets - Definition and detection of ticlopidine/clopidogrel effects. Thrombosis and Haemostasis.82:1145-1152, (scientific article)
- Becker EM, Schmidt P, Schramm M, Schroder H, Walter U, Hoenicka M, Gerzer R, Stasch JP..
(2000). The vasodilator-stimulated phosphoprotein (VASP): Target of YC- 1 and nitric oxide effects in human and rat platelets. Journal of Cardiovascular Pharmacology. 35:390-397, (scientific article)
- Oelze M, Mollnau H, Hoffmann N, Warnholtz A, Bodenschatz M, Smolenski A, Walter U, Skatchkov M, Meinertz T, Munzel T.
(2000). Vasodilator-stimulated phosphoprotein serine 239 phosphorylation as a sensitive monitor of defective nitric oxide/cGMP signaling and endothelial dysfunction. Circulation Research. 87:999-1005, (scientific article)
- Mulsch A, Oelze M, Kloss S, Mollnau H, Topfer A, Smolenski A, Walter U, Stasch JP, Warnholtz A, Hink U, Meinertz T, Munzel T.
(2001). Effects of in vivo nitroglycerin treatment on activity and expression of the guanylyl cyclase and cGMP-dependent protein kinase and their downstream target vasodilator-stimulated phosphoprotein in aorta. Circulation. 103:2188-2194, (scientific article)
- Schulz E, Tsilimingas N, Rinze R, Reiter B, Wendt M, Oelze M, Woelken-Weckmuller S, Walter U, Reichenspurner H, Meinertz T, Munzel T.
(2002). Functional and biochemical analysis of endothelial (dys)function and NO/cGMP signaling in human blood vessels with and without nitroglycerin pretreatment. Circulation 105:1170-1175, (scientific article)
- Mollnau H, Wendt M, Szocs K, Lassegue B, Schulz E, Oelze M, Li HG, Bodenschatz M, August M, Kleschyov AL, Tsilimingas N, Walter U, Forstermann U, Meinertz T, Griendling K, Munzel T.
(2002). Effects of angiotensin II infusion on the expression and function of NAD(P)H oxidase and components of nitric Oxide/cGMP signaling. Circulation Research. 90:E58-E65., (scientific article)
- Aktas B, Utz A, Hoenig-Liedl P, Walter U, Geiger J.
(2002 (in press)). Dipyridamole enhances NO/cGMP mediated VASP phosphorylation and signalling in human platelets: in-vitro and in-vivo/ex-vivo studies. Stroke, (scientific article)
- Smolenski A, Poller W, Walter U, Lohmann SM.
(2000). Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I. Journal of Biological Chemistry. 275:25723-25732, (scientific article)
Links:
Vasopharm Biotech
