Cloning, localisation, and molecular regulation of an inwardly-rectifying potassium channel in migrating cells
Project management at the University of Würzburg:
Migration of transformed renal epithelial cells (MDCK-F cells) depends on
the activity of an inwardly-rectifying calcium-sensitive potassium channel
of intermediate conductance (IK1 channel). This channel supports migration
of MDCK-F cells by induncing a local shrinkage of their rear part. IK1 channel
activity is higher at this cell pole than at the front. One of our aims is
to find out whether the uneven distribution of IK1 channel activity is due
to an uneven distribution of the channel protein in migrating cells.
Therefore, we want to finish cloning of the IK1 channel protein. After
elucidating the structure of the channel we will be able to localize the
channel protein in migrating cells by means of immunocytochemistry.
Moreover, we want to study mechanisms of channel activation by intracellular
Projekt period: from 07.1998 to 06.2000
DFG ,Granting date: 02.02.1998