Leishmania lipophosphoglycan moduliert den Phänotyp und inhibiert die Migration von epidermalen Langerhanszellen
Projektleitung an der Universität Würzburg:
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In this project, the effect of a major parasite surface molecule, Leishmania major lipophosphoglycan (LPG), on the functional activities of dendritic cells was investigated. Epidermal Langerhans cells (LC) are dendritic cells residing in the skin and play a central role in the initiation and regulation of the immune response against the protozoan parasite Leishmania major. LC take up antigens in the skin and transport them to the regional lymph nodes for presentation to T cells. However, it is not known whether LC functions are modulated by parasite moleules. Therefore, we examined the effect of LPG on the maturation of LC and their migratory properties. The results showed that exposure to LPG does not affect the expression of molecules that are important for antigen presentation, MHC class II and B7, but induced an up-regulation of CD25, CD31 and VE-cadherin expression and a down-regulation of Mac-1 expression by LC. Importantly, LPG treatment inhibited the migratory activity of LC, as it reduced their efflux from skin explants and their migration in transwell cultures. These results suggest that Leishmania LPG impairs LC migration out of the skin and, thus, may modulate their immunostimulatory functions, which require LC translocation from skin to lymph nodes.
Laufzeit: von 09.1999 bis 09.2000