Lehrstuhl für Kinderheilkunde
Josef-Schneider-Str.2, 97080 Würzburg
Other participating persons and organisations:
Research foci (and basic equipment-based research projects):
1) Pathogenesis of Lyme arthritis; interaction of
synovial cells with Borrelia burgdorferi:
Borrelia burgdorferi is transmitted to humans by ticks. Lyme disease and especially Lyme arthritis is characterized by inflammatory reactions in the synovial tissue. A presence of viable Borrelia burgdorferi in the joint throughout the disease seems to contribute to the pathogenesis of disease. The interaction of B. burgdorferi with human synovial cells in vitro is analyzed, especially the mechanisms of chemoattraction and activation of leucocytes by human synoviocytes exposed to Borrelia burgdorferi in vitro.
2) Receptor editing in tonsil B cells: Rearrangement of immunoglobulin genes in B cells is taking place during B cell development in the bone marrow. So far this process has not been documented in peripheral lymphoid tissue. However, we could demonstrate significant peripheral receptor editing in human tonsils analyzing RAG1, RAG2 and TdT gene expression.
3) RAG1 and RAG2 gene expression in peripheral B cells of SLE patients:
Rearrangement of immunoglobulin genes in perpheral B cells is limited to bone marrow and peripheral lymphoid tissue. We hypothesized that receptor editing in human SLE patients will take place in B cells of the peripheral blood and that this contributes to the autoimmune B cell pool. We could demonstrate significant receptor editing in human peripheral SLE B cells by analyzing RAG1, RAG2 and TdT gene expression.
4) Receptor editing in peripheral B cells of children
with juvenile rheumatoid arthritis: Anti-nuclear antibodies are the hallmarks of early onset juvenile rheumatoid arthritis and of SLE patients demonstrating autoimmunity. We assume that, as in SLE, receptor editing is activated in human peripheral blood B cells of children with ANA-positive arthritis.
6) Pathogenesis of chronic multifocal osteomyelitis: In a cohort of patients with chronic multifocal non-bacterial osteomyelitis we are evaluating guidelines for diagnosis (microbiology, imaging, histopathology) and therapy of this rare disease.
7) Genotype-phenotype-correlation of infantil-
The pathogenesis of infantile-juvenile hypophosphatasia and particularly the correlation of the genotype with biochemical and clinical characteristics will be investigated.