Architecture of thenuclear periphery of meiotic cells
Project management at the University of Würzburg:
The molecular mechanisms that enable homologous chromosomes to find each other during meiosis are not known. Circumstantial evidence indicates that the nuclear envelope (NE) plays an essential role since the chromosomes interact via their telomeres dynamically with the NE. Better understanding of the dynamic interactions between NE and telomeres would provide a significant progress in our knowledge on the mechanisms of meiosis. We have characterized a meiosis-specific structural protein of the mammalian NE (the A-type lamin C2) as the first protein which is enriched at the attachment sites of meiotic chromosomes. Recently, we were able to show that in Lmna-/- mice lacking A-type lamins, spermatogenesis is disrupted and that spermatocytes show severe defects in synaptic pairing of chromosomes. These results provided evidence for the involvement of the nuclear lamina in meiotic chromosome events and that A-type lamins are an important determinant of male fertility. We were also able to show that NE association of lamin C2 is mediated by the N-terminal myristoylglycine. The involvement of myristoylation in NE association of a member of the lamin family is without precedent in the literature since nuclear envelope association of other members of the lamin family is achieved by isoprenylation of the C-terminus.
Projekt period: from 04.2000 to 03.2002
DFG ( Be 1168/4-3 ) ,Granting date: 28.02.2000
DFG: Be 1168/4-2