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Third-party-funded project

Structures, functions, and assembly of the nuclear pore complexes

Project management at the University of Würzburg:

Participating scientists:

Nuclear pore complexes mediate the transmembrane exchange of macromolecules between the nucleus and the cytoplasm. These bidirectional transport processes are highly selective, saturable and energy-requiring. Knowledge of nuclear pore complex structure, composition and molecular organization is a necessary prerequisite to understanding these transport mechanisms. We are using both monoclonal antibodies against pore complex constituents and autoimmune antibodies from patient sera in order to identify novel members of the nucleoporins. Nuclear pore complexes are dynamic structures. During mitosis the nuclear envelope breaks down into numerous vesicles and reforms at telophase. In order to analyze the mode and order of interaction of the disassembled nuclear envelope and pore complex constituents during the reformation process, we have used a cell free extract. This extract is prepared from eggs of Xenopus laevis and capable of reproducing postmitotic reformation of the nuclear envelope and assembly of pore complexes.

Key words:
    nuclear membrane
    nuclear pore complex
    Xenopus egg extract
    nuclear envelope assembly
    annulate lamellae

Projekt period: from 01.1997 to 12.1999

Funding institution:
DFG ( SFB 176 )