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Third-party-funded project

Molecular analysis of nucleolus assembly

Project management at the University of Würzburg:

Participating scientists:

By using a combination of immunocytochemical and electron microscopical analyses we have studied the functional organization of the amplified nucleoli of Xenopus oocytes. We have developed a panel of antibodies and in situ hybridization probes which are specific for the three major components of functionally active oocyte nucleoli. With these tools at hand we have studied the de novo formation of the amplified nucleoli during early stages of oogenesis. Our results show that nucleoli are generated by a stepwise interaction and fusion process between a highly organized form of the amplified rDNA and preformed nucleolar building blocks. In addition, we have analyzed the organization of the nucleoli of grasshopper (Locusta migratoria) oocytes and have identified structures which most likely represent rRNA transcription units in situ.

Key words:
    amplified nucleoli
    rRNA genes

Projekt period: from 04.1998 to 03.2000

Funding institution:
DFG ( Sche 157/12-1 ) ,Granting date: 12.03.1998

Preceding project:
DFG: Sche 157/10-1