research report      name index      key word index      corresp.unit            Page in german      Imprint + Privacy Policy   

Third-party-funded project

Title:
Molecular analysis of nucleolus assembly

Project management at the University of Würzburg:

Participating scientists:

Abstract:
By using a combination of immunocytochemical and electron microscopical analyses we have studied the functional organization of the amplified nucleoli of Xenopus oocytes. We have developed a panel of antibodies and in situ hybridization probes which are specific for the three major components of functionally active oocyte nucleoli. With these tools at hand we have studied the de novo formation of the amplified nucleoli during early stages of oogenesis. Our results show that nucleoli are generated by a stepwise interaction and fusion process between a highly organized form of the amplified rDNA and preformed nucleolar building blocks. In addition, we have analyzed the organization of the nucleoli of grasshopper (Locusta migratoria) oocytes and have identified structures which most likely represent rRNA transcription units in situ.

Key words:
    Xenopus
    Locusta
    oocytes
    amplified nucleoli
    UBF
    rRNA genes

Projekt period: from 04.1998 to 03.2000

Funding institution:
DFG ( Sche 157/12-1 ) ,Granting date: 12.03.1998

Preceding project:
DFG: Sche 157/10-1

Publications: