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Third-party-funded project

Title:
Lampbrush chromosomes as a model system for transcriptionally active chromatin

Project management at the University of Würzburg:

• Prof. Dr. Dr. Ulrich Scheer

Participating scientists:

• Dr. Robert Hock
• Dipl. Biol. Ulrich Körner

Abstract:
Lampbrush chromosomes of amphibian oocytes present a valuable model system since they allow the direct light microscopic visualization of transcriptionally active genes in the form of lateral loops. Our studies are aimed at the identification of the protein compositon of these highly active genes as compared to that of the inactive chromosome axis. Furthermore, we want to gain some more insight into the structural organization and function of the RNA transcripts of the lampbrush chromosomes.

Key words:
    lampbrush chromosomes
    amphibian oocyte
    DNA topoisomerase
    maternal RNA

Projekt period: from 01.1996 to 12.2002

Funding institution:
Sonstige öffentliche Mittel ( Haushalt Lehrstuhl )

Publications:

• Hock, R., M. Carl, B. Lieb, D. Gebauer and U. Scheer. (1996). A monoclonal antibody against DNA topoisomerase II labels the axial granules of Pleurodeles lampbrush chromosomes. (monograph)
• Gebauer, D., C. Mais, K. Zinger, R. Hock, B. Lieb and U. Scheer. (1996). Localization of a high molecular weight form of DNA topoisomerase I in amphibian oocytes.. (monograph)
• Lieb, B., M. Carl, R. Hock, D. Gebauer and U. Scheer. (1998). Identification of a novel mRNA-associated protein in oocytes of Pleurodeles walt and Xenopus laevis. (monograph)
• Stöck, M., D.K. Lamatsch, C. Steinlein, J.T. Epplen, W.-R. Grosse, R. Hock, T. Klapperstück, K.P. Lampert, U. Scheer, M. Schmidt and M. Schartl. (2002). A bisexually reproducing all-triploid vertebrate. (monograph)