Expression of transcription factors used by RNA polymerase I during early amphibian embryogenesis
Project management at the University of Würzburg:
Transcription of the rRNA genes and production of ribosomes begins not until the early gastrula stage of Xenopus embryos. Up to this developmental stage protein biosynthesis of the embryo is based entirely on maternally provided ribosomes. The molecular mechanism which trigger the zygotic gene expression are as yet poorly understood. In collaboration with the group of Brian McStay (Dundee, Scotland) we are studying the expression and localization of factors which are essential for transcripton of the rRNA genes. Presently our focus is on the transcripton factor TAF63 which -together with other proteins- forms the multi-subunit transcription complex SL1. We have already determined the amino acid sequence of Xenopus TAF63 and could show by using RT-PCR that maternal transcripts are present in oocytes and throughout embryogenesis. After raising specific antibodies we plan to examine the expression of TAF63 at the protein level and the time course of interactions with the promotor of the rRNA genes.
Projekt period: from 12.2001 to 06.2003
Sonstige öffentliche Mittel ( Haushalt Lehrstuhl )