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Third-party-funded project

Title:
Genotoxicity of Dioxetanes and Photo-Fenton Reagents

Project management at the University of Würzburg:

• Prof. Dr. Dr. h.c. Waldemar Adam ,Tel: + 49 (0)931 8885334,Fax: + 49 (0)931 8884756,Mail: adam@chemie.uni-wuerzburg.de

Participating scientists:

• Dipl.-Chem. Simone Andler
• Dipl.-Chem. Markus Arnold
• Dipl.-Chem. Günther Grimm
• Dipl.-Chem. Annemarie Kurz
• Dipl.-Chem. Stefan Marquardt
• Dipl.-Chem. Karsten Mielke
• Dr. Marianne Möller
• Dr. Chantu Saha-Möller ,Tel: + 49 (0)931 8884752,Fax: + 49 (0)931 8884756,Mail: sahamoel@chemie.uni-wuerzburg.de

Abstract:
The chemical, analytical, mechanistic, and biochemical investigations of oxidative DNA damage caused by electronically excited species and oxyl radicals are the main objectives of this multidisciplinary research project in the field of photobiology. The necessary electronically excited species, in particular carbonyl compounds, are generated by direct irradiation of respective ketones or by thermal decomposition of dioxetanes, while oxyl radicals are produced photochemically from photo-Fenton reagents.

Key words:
    Photobiology
    Genotoxicity
    Cytotoxicity
    Mutagenicity
    DNA Photooxidation
    DNA Strand Break
    Dioxetane
    Electronically Excited Species
    Oxyl Radical
    Photo-Fenton Reagent

Projekt period: from 01.1997 to 12.1999

Funding institution:
DFG ,Granting date: 19.12.1996

Preceding project:
SFB 172 vierte Projektperiode

Publications:

• Adam, W.; Arnold, M: A.; Nau, W.; Pischel, U.; Saha-Möller, C. R. (2002). A Comparative Photomechanistic Study (Spin Trapping, EPR Sectroscopy, Transient Kinetics, Photoproducts) of Nucleoside Oxidation (dG and 8-oxodG) by Triplet-Excited Acetophenones and by the Radicals Generated from α-Oxy-Substituted Derivatives through Norrish-Type I Cleavage. J. Am. Chem. Soc., 124, 3893 - 3904, (scientific article)
• Adam, W.; Marquardt, S.; Kemmer, D.; Saha-Möller, C. R.; Schreier, P.. (2002). 2'- Deoxyguanosin (dG) Oxidation and Strand-Break Formation in DNA by the Radicals Released in the Photolysis of N-tert- Butoxy-2-pyridone. Are tert-Butoxyl or Methyl Radicals for the Photooxidative Damage in Aqueous Media?. Org. Lett., 2002, 225 - 228, (scientific article)
• Adam, W.; Arnold, M. A.; Saha-Möller, C. R.. (2001). Photooxidative Damage of Guanine in dG and DNA by the Radicals Drived from the α Cleavage of the Electronically Exicted Carbonyl Products Generated in the Thermolysis of Alkoxymethyl-Substituted Dioxetanes and the Photolysis of Alkoxyacetones. J. Org. Chem., 66, 597 - 604, (scientific article)
• Adam, W.; Kurz, A.; Saha-Möller C. R.. (1999). DNA and 2'-Deoxyguanosine Damage in the Horseradish-Peroxidase-Catalyzed Autoxidation of Aldehydes: The Search for the Oxidizing Species. Free Radic. Biol. Med., 26, 566 - 579, (scientific article)
• Adam, W.; Grimm, G. N.; Marquardt, S.; Saha-Möller, C. R.. (1999). Are Pyridinethiones Reliable Photochemical Oxyl-Radical Sources for Photobiological Studies? The Importance of Secondary Photolysis Products in the Guanine Oxidation of 2'-Deoxyguanine and Cell-Free DNA. J. Am. Chem. Soc., 121, 1179 - 1185, (scientific article)
• Adam, W.; Andler, S.; Nau, W. M.; Saha-Möller, C. R.. (1998). Oxidative DNA Damage by Radicals Generated in the Thermolysis of Hydroxymethyl-Substituted 1,2-Dioxetanes through the α Cleavage of Chemiexcited Ketones. J Am. Chem. Soc., 120, 3549 - 3559, (scientific article)

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