Lehrstuhl für Organische Chemie II
Am Hubland, 97074 Würzburg
Other participating persons and organisations:
Research foci (and basic equipment-based research projects):
Oxidative DNA Damage by Electronically Excited States and Reactive Oxygen Species:
The research interests in this area concern the genotoxicity and cytotoxicity of electronically excited species, particularly triplet-excited carbonyl compounds and oxyl radicals. The necessary triplet states are generated either photochemically by direct irradiation of suitable ketones or thermally from dioxetanes (high-energy molecules), while oxyl radicals are produced photochemically from the so-called photo-Fenton reagents. The genotoxicity studies are performed with calf thymus DNA or supercoiled plasmid DNA, and the cytotoxicity is assessed mainly in mouse lymphoma cells. For the mechanistic elucidation of oxidative DNA damage, primarily 2'-deoxyguanosine is used as model substrate.
For the results in this research area, consult the above-mentioned publications.
Rewards and prizes:
Dr. D. Kazakov: Alexander-von-Humboldt Fellowship, 2002.
Dr. H. Okamoto: Research Fellowship of Japanese Government.
EPR Spectrometer, Fluorescence Spectrometer, Gel-Electrophoresis System, HPLC, Electrochemical Detector, Fluorescence Detector, MS, NMR Spectrometers (600, 400, 250 and 200 MHz), Rayonet Photoreactor, UV Spectrophotometer.