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Third-party-funded project

Title:
Biochemical studies of the alpha-oxidation in pea (Pisum sativum)

Project management at the University of Würzburg:

• Prof. Dr. Peter Schreier ,Tel: 0931-8885481,Fax: 0931-8885484,Mail: schreier@pzlc.uni-wuerzburg.de

Participating scientists:

• Priv. Doz. Dr. Astrid Schön
• Dr. Jenny Hartmann-Schreier
• Dr. Michael Lazarus
• Dr. Alexander Saffert

Abstract:
An enzyme with fatty acid alpha-oxidation activity was purified from germinating pea (Pisum sativum) to apparent electrophoretic homogeneity. The purified protein was found to be a 230 kD oligomer with to subunits, i.e. a 50 kD subunit with NAD oxidoreductase activity and a 70 kD subunit, homolog to a pathogen-induced oxygenase (PIOX). LC-ESI-MS/MS analysis revealed rapid alpha-oxidation of palmitic acid incubated with the 70 kD subunit, leading to (R)-2-hydroperoxypalmitic acid as the major product together with (R)-2-hydroxypalmitic acid, 1-pentadecanal, and pentadecanoic acid. The kinteic data af a number of substrates were determined.

Key words:
    alpha-Oxidase
    PIOX
    2-Hydroperoxy fatty acids
    Pisum sativum
    Dioxygenase
    HPLC-MS/MS

Projekt period: from 01.1998 to 12.2000

Funding institution:
DFG ,Granting date: 01.10.1997

Publications:

• W. Adam, M. Lazarus, C.R. Saha-Möller, P. Schreier. (1996). Enantioselective alpha-hydroxylation of carboxylic acids with molecular oxygen catalyzed by the alpha-oxidation system of young pea leaves (Pisum sativum): A substrate selectivity study. (monograph)
• A. Saffert, J. Hartmann-Schreier, A. Schön, P. Schreier. (2000). A dual function aalpha-dioxygenase-peroxidase and NAD oxidoreductase active enzyme from germinating pea rationalizing alpha-oxidation of fatty acids in plants. (monograph)

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