Research focus: |
Lehrstuhl für Physikalische Chemie II
Am Hubland, 97074/Würzburg Mail: wolfgang.kiefer@mail.uni-wuerzburg.de Url: http://www.phys-chemie.uni-wuerzbur.de/kiefer/welcome.html |
Scientific members:
Professors:
Outside lecturer:
Scientific assistants:
Other participating persons and organisations:
Research foci (and basic equipment-based research projects):
During the last couple of years in-vivo Raman spectroscopical investigations of biological tissues and cells became of siginificant importance in the fields of medical, pharmaceutical and biological diagnosis. In this context the chemical composition of cell material and its dependency on exogenous factors are of utmost importance. Therefore it must be the task of in-vivo methods to provide these information about the cell ingredients of living cells without destroying them. With the aid of micro Raman spectroscopy which is a combination of a light optical microscope and a conventional Raman apparatus it is possible to resolve textures up to the submicron range and to record Raman spectra with such a spatial resolution. With this technique Raman spectra of single cells, cell organelles or special texture areas can be obtained. Furthermore it is possible to directly localize and identify biological relevant molecules in a living plant.
Results:
For the results in this research area, please consult the above-mentioned publications.
Rewards and prizes:
Prof. Dr. W. Kiefer: Honorary Fellow of the Laser and Spectroscopy Society of India (F.L.S.S.), 1999. - Distinguished Service Award of the Society of Applied Spectroscopy, 2000. - Foreign Councillor of the Institute for Molecular Science, Okazaki National Research Institutes, Okazaki, Japan, 1999-2001. - Technical Expert Monitor of the International Science and Technology Center, Moscow, Russia, 1999-2002. - Chief Editor of the: Journal of Raman Spectroscopy, 2000. -- PD Dr. J. Popp: Zehetmaier Award (postdoctoral lecture qualification scholarship) awarded by the Freistaat Bayern, 1999. - Cooperation Prize of the University of Würzburg, 2001, - Bunsen-Kirchhoff Prize awarded by the German Bunsen-Society, 2002.
Equipment:
Micro Raman set up incl. confocal pinhole (LabRam JobinYvon): HeNe-Laser, 785 diode laser, Ar ion laser (514 nm) and cw Nd:YAG laser / accessories; VIS Raman spectrometer incl. microscope: Ar ion laser / XY triple monochromator (DILOR) / OMA detector; VIS Raman spectrometer incl. microscope: Kr ion laser + Ar ion laser / Spex 1404 double monochromator / CCD camera; UV/VIS Raman spectrometer incl. microscope: Ar ion laser (275, 300, 305, 333, 351, 363, 454, 457, 466, 473, 476, 488, 496 and 514 nm) / Spex 1404 double monochromator / CCD camera; NIR Raman spectrometer: cw Nd:YAG laser / Jarrell Ash spectrometer / InGaAs detector / lock in amplifier; FT-IR spectrometer incl. FT-Raman module (Bruker): Nd:YAG laser / Ge detector / accessories.