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Research focus:  

SFB 355 Pathophysiologie der Herzinsuffizienz Medizinische Klinik
Med. Universitätsklinik, Josef-Schneider-Str. 2, D-97080 Würzburg

Scientific members:


   Outside lecturer:

   Scientific assistants:

Research foci (and basic equipment-based research projects):
Collaborative Research Centre 355: Pathophysiology of Heart Failure

The central theme of the SFB 355 is the study of the causes, consequences, and therapy of heart failure. The heart failure syndrome results primarily from heart and circulatory diseases such as coronary heart disease, myocardial infarction, hypertension, and has a major medical and socio-economic impact due to its increasing incidence. The multifactorial etiology and complex consequences for other organ systems requires an interdisciplinary research approach. Research groups of section A (Nuclear Magnetic Resonance) of the SFB 355 develop new NMR methods for analysis of cardiac morphology, circulation, metabolism, and function. These methods are applied a) to pathophysiological mechanisms of heart failure, and b) to clinical studies of patients treated for heart failure. Section B (Pathophysiology) investigates the basic mechanisms of development of chronic heart failure after myocardial infarction, especially the role of endogenous hormones (renin, angiotensin, endothelin, atrial factors, and nitric oxide), endothelial cells, and important regulators of calcium homeostasis. Section C (Pathobiochemistry and Molecular Biology) focuses on analyses of cell and molecular biology of the functions of receptors for hormones as well as adhesion and growth factors, and their significance with respect to heart failure. Furthermore, genetically engineered transgenic mice are being developed and examined as animal models of heart disease in the Würzburg SFB 355 using the recently developed methods in biochemistry, pharmacology, physiology, and physics.

SFB 355 Publications 1999: Articles

(A1-C10: Projects involved)

1. Deichmann, R., Hahn, D. and Haase, A. (1999a) Fast T1 mapping on a whole-body scanner. Magn Reson Med, 42, 206-9. (A1)

2. Deichmann, R., Wiesmann, F., Hillenbrand, C., Hahn, D. and Haase, A. (1999b) Contrast enhancement and artifact reduction in magnetization-prepared MR angiography. Int J of Imaging Systems & Techn, 10, 266-272. (A1)

3. Hillenbrand, C., Deichmann, R., Hahn, D. and Haase, A. (1999) Signal intensities in FLASH-EPI-hybrid sequences. J Magn Reson, 139, 74-80. (A1)

4. Kahler, E., Waller, C., Rommel, E., Belle, V., Hiller, K.H., Voll, S., Bauer, W.R. and Haase, A. (1999) Perfusion-corrected mapping of cardiac regional blood volume in rats in vivo. Magn Reson Med, 42, 500-6. (A1, A6, A8)

5. Sandstede, J.J., Bertsch, G., Beer, M., Kenn, W., Werner, E., Pabst, T., Lipke, C., Kretschmer, S., Neubauer, S. and Hahn, D. (1999a) Detection of myocardial viability by low-dose dobutamine Cine MR imaging. Magn Reson Imaging, 17, 1437-43. (A1, A3)

6. Sandstede, J.J., Pabst, T., Beer, M., Geis, N., Kenn, W., Neubauer, S. and Hahn, D. (1999b) Three-dimensional MR coronary angiography using the navigator technique compared with conventional coronary angiography. AJR Am J Roentgenol, 172, 135-9. (A1, A3)

7. Sandstede, J., Lipke, C., Beer, M., Harre, K., Pabst, T., Kenn, W., Neubauer, S. and Hahn, D. (1999) CINE-MRT for the study of the effects of regional left ventricular wall motion disorders on global heart function after a myocardial infarct and revascularization. Rofo Fortschr Geb Rontgenstr Neuen Bildgeb Verfahr, 171, 424-30. (TP A1, A3)

8. Hugel, S., Horn, M., de Groot, M., Remkes, H., Dienesch, C., Hu, K., Ertl, G. and Neubauer, S. (1999a) Effects of ACE inhibition and beta-receptor blockade on energy metabolism in rats postmyocardial infarction. Am J Physiol, 277, H2167-75. (A3, B9)

9. Horn, M., Remkes, H., Dienesch, C., Hu, K., Ertl, G. and Neubauer, S. (1999) Chronic high-dose creatine feeding does not attenuate left ventricular remodeling in rat hearts post-myocardial infarction. Cardiovasc Res, 43, 117-24. (A3, B9)

10. Neubauer, S., Hu, K., Horn, M., Remkes, H., Hoffmann, K.D., Schmidt, C., Schmidt, T.J., Schnackerz, K. and Ertl, G. (1999a) Functional and energetic consequences of chronic myocardial creatine depletion by beta-guanidinopropionate in perfused hearts and in intact rats. J Mol Cell Cardiol, 31, 1845-55. (A3, B9)

11. Neubauer, S., Remkes, H., Spindler, M., Horn, M., Wiesmann, F., Prestle, J., Walzel, B., Ertl, G., Hasenfuss, G. and Wallimann, T. (1999b) Downregulation of the Na(+)-creatine cotransporter in failing human myocardium and in experimental heart failure. Circulation, 100, 1847-50. (A3, A6, B9)

12. Hugel, S., Neubauer, S., Lie, S.Z., Ernst, R., Horn, M., Schmidt, H.H., Allolio, B. and Reincke, M. (1999b) Multiple mechanisms are involved in the acute vasodilatory effect of 17beta-estradiol in the isolated perfused rat heart. J Cardiovasc Pharmacol, 33, 852-8. (A3, C7)

13. Hugel, S., Reincke, M., Stromer, H., Winning, J., Horn, M., Dienesch, C., Mora, P., Schmidt, H.H., Allolio, B. and Neubauer, S. (1999c) Evidence against a role of physiological concentrations of estrogen in post-myocardial infarction remodeling. J Am Coll Cardiol, 34, 1427-34. (A3, C7)

14. Pohmann, R., Rommel, E. and von Kienlin, M. (1999) Beyond k-Space: Spectral Localization Using Higher Order Gradients. J Magn Reson, 141, 197-206. (A7)

15. Beer, M., Landschutz, W., Meininger, M., Seyfarth, T., Viehrig, M., Sandstede, J., Pabst, T., Kenn, W., Horn, M., Harre, K., von Kienlin, M., Neubauer, S. and Hahn, D. (1999) Determination of absolute concentrations of cardiac high-energy phosphates using 31P-MR-spectroscopy and SLOOP in healthy and diseased myocardium. Rofo Fortschr Geb Rontgenstr Neuen Bildgeb Verfahr, 171, 65-8. (A7, A3, A1)

16. Meininger, M., Landschutz, W., Beer, M., Seyfarth, T., Horn, M., Pabst, T., Haase, A., Hahn, D., Neubauer, S. and von Kienlin, M. (1999) Concentrations of human cardiac phosphorus metabolites determined by SLOOP 31P-NMR- spectroscopy. Magn Reson Med, 41, 657-63. (A7, A3, A1)

17. Wacker, C.M., Bock, M., Hartlep, A.W., Beck, G., van Kaick, G., Ertl, G., Bauer, W.R. and Schad, L.R. (1999b) Changes in myocardial oxygenation and perfusion under pharmacological stress with dipyridamole: assessment using T*2 and T1 measurements. Magn Reson Med, 41, 686-95. (A8)

18. Wacker, C.M., Bock, M., Hartlep, A.W., Bauer, W.R., van Kaick, G., Pfleger, S., Ertl, G. and Schad, L.R. (1999a) BOLD-MRI in ten patients with coronary artery disease: evidence for imaging of capillary recruitment in myocardium supplied by the stenotic artery. Magma, 8, 48-54. (A8, B9)

19. Bauer, W.R., Nadler, W., Bock, M., Schad, L.R., Wacker, C., Hartlep, A. and Ertl, G. (1999b) Theory of the BOLD effect in the capillary region: an analytical approach for the determination of T2 in the capillary network of myocardium. Magn Reson Med, 41, 51-62. (A8, B9)

20. Bauer, W.R., Nadler, W., Bock, M., Schad, L.R., Wacker, C., Hartlep, A., and Ertl, G. (1999) Theory of coherent and incoherent nuclear spin dephasing in the heart. Phys Rev Letters, 83, 4215-18. (A8, B9)

21. Bauer, W.R., Nadler, W., Bock, M., Schad, L.R., Wacker, C., Hartlep, A. and Ertl, G. (1999a) The relationship between the BOLD-induced T(2) and T(2)(*): A theoretical approach for the vasculature of myocardium. Magn Reson Med, 42, 1004-1010. (A8, B9)

22. Gudi, T., Hong, G.K., Vaandrager, A.B., Lohmann, S.M. and Pilz, R.B. (1999) Nitric oxide and cGMP regulate gene expression in neuronal and glial cells by activating type II cGMP-dependent protein kinase. Faseb J, 13, 2143-52. (B4)

23. Hoenderop, J.G., Vaandrager, A.B., Dijkink, L., Smolenski, A., Gambaryan, S., Lohmann, S.M., de Jonge, H.R., Willems, P.H. and Bindels, R.J. (1999) Atrial natriuretic peptide-stimulated Ca2+ reabsorption in rabbit kidney requires membrane-targeted, cGMP-dependent protein kinase type II. Proc Natl Acad Sci U S A, 96, 6084-9. (B4)

24. Hauser, W., Knobeloch, K.P., Eigenthaler, M., Gambaryan, S., Krenn, V., Geiger, J., Glazova, M., Rohde, E., Horak, I., Walter, U. and Zimmer, M. (1999) Megakaryocyte hyperplasia and enhanced agonist-induced platelet activation in vasodilator-stimulated phosphoprotein knockout mice. Proc Natl Acad Sci U S A, 96, 8120-5. (B4, C3, C8)

25. Sporbert, A., Mertsch, K., Smolenski, A., Haseloff, R.F., Schonfelder, G., Paul, M., Ruth, P., Walter, U. and Blasig, I.E. (1999) Phosphorylation of vasodilator-stimulated phosphoprotein: a consequence of nitric oxide- and cGMP-mediated signal transduction in brain capillary endothelial cells and astrocytes. Brain Res Mol Brain Res, 67, 258-66. (B4, C8)

26. Benkwitz, C., Oberdorf-Maass, S. and Neyses, L. (1999) Combined SSCP and heteroduplex analysis of the human plasma membrane Ca(2+)-ATPase isoform 1 in patients with essential hypertension. Biochem Biophys Res Commun, 261, 515-20. (B6)

27. Shamim, A., Pelzer, T., Grohe, C. and Neyses, L. (1999) Induction of Egr-1 mRNA and protein by endothelin 1, angiotensin II and norepinephrine in neonatal cardiac myocytes. Mol Cell Biochem, 195, 11-7. (B6)

28. Jacobs, M., Staufenberger, S., Gergs, U., Meuter, K., Brandstatter, K., Hafner, M., Ertl, G. and Schorb, W. (1999) Tumor necrosis factor-alpha at acute myocardial infarction in rats and effects on cardiac fibroblasts. J Mol Cell Cardiol, 31, 1949-59. (TP B9)

29. Bauersachs, J., Bouloumie, A., Fraccarollo, D., Hu, K., Busse, R. and Ertl, G. (1999) Endothelial dysfunction in chronic myocardial infarction despite increased vascular endothelial nitric oxide synthase and soluble guanylate cyclase expression: role of enhanced vascular superoxide production. Circulation, 100, 292-8. (B9, B10)

30. Aulbach, F., Simm, A., Maier, S., Langenfeld, H., Walter, U., Kersting, U. and Kirstein, M. (1999) Insulin stimulates the L-type Ca2+ current in rat cardiac myocytes. Cardiovasc Res, 42, 113-20. (C2, C8)

31. Maier, S., Aulbach, F., Simm, A., Lange, V., Langenfeld, H., Behre, H., Kersting, U., Walter, U. and Kirstein, M. (1999) Stimulation of L-type Ca2+ current in human atrial myocytes by insulin. Cardiovasc Res, 44, 390-7. (C2, C8)

32. Schwarz, U.R., Geiger, J., Walter, U. and Eigenthaler, M. (1999) Flow cytometry analysis of intracellular VASP phosphorylation for the assessment of activating and inhibitory signal transduction pathways in human platelets--definition and detection of ticlopidine/clopidogrel effects. Thromb Haemost, 82, 1145-52. (C3, C8)

33. Golenhofen, N., Htun, P., Ness, W., Koob, R., Schaper, W. and Drenckhahn, D. (1999) Binding of the stress protein alpha B-crystallin to cardiac myofibrils correlates with the degree of myocardial damage during ischemia/reperfusion in vivo. J Mol Cell Cardiol, 31, 569-80. (C6)

34. Ruetten, H., Zabel, U., Linz, W. and Schmidt, H.H. (1999) Downregulation of soluble guanylyl cyclase in young and aging spontaneously hypertensive rats. Circ Res, 85, 534-41. (C7)

35. Seeber, F., Beuerle, B. and Schmidt, H.H. (1999) Cloning and functional expression of the calmodulin gene from Toxoplasma gondii. Mol Biochem Parasitol, 99, 295-9. (C7)

36. Usunoff, K.G., Kharazia, V.N., Valtschanoff, J.G., Schmidt, H.H. and Weinberg, R.J. (1999) Nitric oxide synthase-containing projections to the ventrobasal thalamus in the rat. Anat Embryol (Berl), 200, 265-81. (C7)

37. Zabel, U., Hausler, C., Weeger, M. and Schmidt, H.H. (1999) Homodimerization of soluble guanylyl cyclase subunits. Dimerization analysis using a glutathione s-transferase affinity tag. J Biol Chem, 274, 18149-52. (C7)

38. Feelisch, M., Kotsonis, P., Siebe, J., Clement, B. and Schmidt, H.H. (1999) The soluble guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3,-a] quinoxalin-1-one is a nonselective heme protein inhibitor of nitric oxide synthase and other cytochrome P-450 enzymes involved in nitric oxide donor bioactivation. Mol Pharmacol, 56, 243-53. (C7)

39. Frey, A., Schneider-Rasp, S., Marienfeld, U., Yu, J.C., Paul, M., Poller, W. and Schmidt, H.H. (1999) Biochemical and functional characterization of nitric oxide synthase III gene transfer using a replication-deficient adenoviral vector. Biochem Pharmacol, 58, 1155-66. (C7)

40. Frohlich, L.G., Kotsonis, P., Traub, H., Taghavi-Moghadam, S., Al-Masoudi, N., Hofmann, H., Strobel, H., Matter, H., Pfleiderer, W. and Schmidt, H.H. (1999) Inhibition of neuronal nitric oxide synthase by 4-amino pteridine derivatives: structure-activity relationship of antagonists of (6R)- 5,6,7,8-tetrahydrobiopterin cofactor. J Med Chem, 42, 4108-21. (C7)

41. Galle, J., Zabel, U., Hubner, U., Hatzelmann, A., Wagner, B., Wanner, C. and Schmidt, H.H. (1999) Effects of the soluble guanylyl cyclase activator, YC-1, on vascular tone, cyclic GMP levels and phosphodiesterase activity. Br J Pharmacol, 127, 195-203. (C7)

42. Kotsonis, P., Frey, A., Frohlich, L.G., Hofmann, H., Reif, A., Wink, D.A., Feelisch, M. and Schmidt, H.H. (1999) Autoinhibition of neuronal nitric oxide synthase: distinct effects of reactive nitrogen and oxygen species on enzyme activity. Biochem J, 340, 745-52. (C7)

43. Stopper, H., Moller, M., Bommel, H.M. and Schmidt, H.H. (1999) Cytotoxic versus genotoxic effects of nitric oxide (NO). Toxicol Lett, 106, 59-67. (C7)

44. Reif, A., Frohlich, L.G., Kotsonis, P., Frey, A., Bommel, H.M., Wink, D.A., Pfleiderer, W. and Schmidt, H.H. (1999) Tetrahydrobiopterin inhibits monomerization and is consumed during catalysis in neuronal NO synthase. J Biol Chem, 274, 24921-9. (C7)

45. Jahns, R., Boivin, V., Siegmund, C., Boege, F., Lohse, M.J. and Inselmann, G. (1999a) Activating beta-1-adrenoceptor antibodies are not associated with cardiomyopathies secondary to valvular or hypertensive heart disease. J Am Coll Cardiol, 34, 1545-51. (C9)

46. Zuurmond, H.M., Hessling, J., Bluml, K., Lohse, M. and Ijzerman, A.P. (1999) Study of interaction between agonists and asn293 in helix VI of human beta(2)-adrenergic receptor. Mol Pharmacol, 56, 909-16. (C9)

47. Jahns, R., Boivin, V., Siegmund, C., Inselmann, G., Lohse, M.J. and Boege, F. (1999b) Autoantibodies activating human beta1-adrenergic receptors are associated with reduced cardiac function in chronic heart failure. Circulation, 99, 649-54. (C9)

48. Altman, J.D., Trendelenburg, A.U., MacMillan, L., Bernstein, D., Limbird, L., Starke, K., Kobilka, B.K. and Hein, L. (1999) Abnormal regulation of the sympathetic nervous system in alpha2A- adrenergic receptor knockout mice. Mol Pharmacol, 56,154-61. (C10)

49. Hein, L., Altman, J.D. and Kobilka, B.K. (1999a) Two functionally distinct alpha2-adrenergic receptors regulate sympathetic neurotransmission. Nature, 402, 181-4. (C10)

50. Hein, L., Limbird, L.E., Eglen, R.M. and Kobilka, B.K. (1999b) Gene substitution/knockout to delineate the role of alpha 2- adrenoceptor subtypes in mediating central effects of catecholamines and imidazolines. Ann N Y Acad Sci, 881, 265-71. (C10)

51. Hutchinson, H.G., Hein, L., Fujinaga, M. and Pratt, R.E. (1999) Modulation of vascular development and injury by angiotensin II. Cardiovasc Res, 41, 689-700. (C10)

52. Engelhardt, S., Hein, L., Wiesmann, F. and Lohse, M.J. (1999) Progressive hypertrophy and heart failure in beta1-adrenergic receptor transgenic mice. Proc Natl Acad Sci U S A, 96, 7059-64. (C9, A6)

SFB 355 Publications 2000: Articles

(A1-C10: projects involved)

1. Hahn, D., Beer, M. and Sandstede, J. (2000) Magnetic resonance tomography of the heart. Radiologe, 40, 888-96. (TP A1)

2. Hillenbrand, C., Sandstede, J., Pabst, T., Hahn, D., Haase, A. and Jakob, P.M. (2000b) Hybrid cardiac imaging with MR-CAT scan: a feasibility study. J Magn Reson Imaging, 11, 711-6. (TP A1)

3. Nittka, M. and Haase, A. (2000) Investigation of complex phased array coil designs for cardiac imaging. Magma, 10, 122-30. (TP A1)

4. Ruff, J., Wiesmann, F., Lanz, T. and Haase, A. (2000) Magnetic resonance imaging of coronary arteries and heart valves in a living mouse: techniques and preliminary results Magn Reson, 146, 290-6. (TP A1)

5. H. Köstler, W. Landschütz, J. Sandstede, C. Lipke, D. Hahn. Heart Perfusion Imaging using SENSE. Supplement to Radiology, November 2000, Vol 217 (TP A1)

6. Sandstede, J., Lipke, C., Beer, M., Hofmann, S., Pabst, T., Kenn, W., Neubauer, S. and Hahn, D. (2000a) Age- and gender-specific differences in left and right ventricular cardiac function and mass determined by cine magnetic resonance imaging. Eur Radiol, 10, 438-42. (TP A1, A3)

7. Sandstede, J., Lipke, C., Beer, M., Kenn, W., Pabst, T., Neubauer, S. and Hahn, D. (2000b) Delayed enhancement after injection of Gd-DTPA in dysfunctional myocardium – correlation with regional wall motion improvement after revascularization. Radiologe, 40, 150-4. (TP A1, A3)

8. Sandstede, J., Pabst, T., Beer, M., Harre, K., Baurle, K., Lipke, C., Butter, F., Kenn, W., Volker, W., Neubauer, S. and Hahn, D. (2000c) 23Na MRI for imaging of myocardial infarction in humans. Rofo Fortschr Geb Rontgenstr Neuen Bildgeb Verfahr, 172, 739-43. (TP A1, A3)

9. Sandstede, J.J., Beer, M., Hofmann, S., Lipke, C., Harre, K., Pabst, T., Kenn, W., Neubauer, S. and Hahn, D. (2000d) Changes in left and right ventricular cardiac function after valve replacement for aortic stenosis determined by cine MR imaging. J Magn Reson Imaging, 12, 240-6. (TP A1, A3)

10. Sandstede, J.J., Lipke, C., Beer, M., Harre, K., Pabst, T., Kenn, W., Neubauer, S. and Hahn, D. (2000e) Analysis of first-pass and delayed contrast-enhancement patterns of dysfunctional myocardium on MR imaging: use in the prediction of myocardial viability. AJR Am J Roentgenol, 174, 1737-40. (TP A1, A3)

11. Wiesmann, F., Ruff, J., Hiller, K.H., Rommel, E., Haase, A. and Neubauer, S. (2000) Developmental changes of cardiac function and mass assessed with MRI in neonatal, juvenile, and adult mice. Am J Physiol Heart Circ Physiol, 278, H652-7. (TP A1, A3, A6)

12. Beer, M., Sandstede, J., Landschutz, W., Viehrig, M., Harre, K., Horn, M., Meininger, M., Pabst, T., Kenn, W., Haase, A., von Kienlin, M., Neubauer, S. and Hahn, D. (2000a) Altered energy metabolism after myocardial infarction assessed by 31P-MR-spectroscopy in humans. Eur Radiol, 10, 1323-8. (TP A1, A3, A7)

13. Weidensteiner, C., Lanz, T., Horn, M., Neubauer, S., Haase, A. and von Kienlin, M. (2000) Three-dimensional (13)C-spectroscopic imaging in the isolated infarcted rat heart. J Magn Reson, 143, 17-23. (TP A1, A3, A7)

14. Beer, M., Viehrig, M., Seyfarth, T., Sandstede, J., Lipke, C., Pabst, T., Kenn, W., Harre, K., Horn, M., Landschutz, W., von Kienlin, M., Neubauer, S. and Hahn, D. (2000b) Analysis of cardiac energy metabolism in valve disease using 31P-MR-spectroscopy. Radiologe, 40, 162-7. (TP A1, A3, A7)

15. Haase A. , F. Odoj, M. von Kienlin, J. Warnking, F. Fidler, A. Weisser, M. Nittka, E. Rommel, T. Lanz, B. Kalusche, M. Griswold. (2000) NMR Probeheads for In Vivo Applications; Concepts Magn. Reson., 12: 361-388 (TP A1, A6, A7)

16. Nahrendorf M, Wiesmann F, Hiller K-H, Han H, Hu K, Waller C, Ruff J, Haase A, Ertl G, Bauer W: In vivo assessment of cardiac remodeling after myocardial infarction in rats by cine-magnetic resonance imaging. JCMR 2000; 2:171-180. (TP A1, A6, A8, B9)

17. Wiesmann F, Ruff J, Dienesch C, Leupold A, Rommel E, Haase A, Neubauer S: Cardiovascular phenotype characterisation in mice by high resolution magnetic resonance imaging. MAGMA 2000 (TP A1, A3, A6)

18. Hillenbrand, C., Hahn, D., Haase, A. and Jakob, P.M. (2000a) MR CAT scan: a modular approach for hybrid imaging. Magma, 10, 183-99. (TP A1, A7)

19. Seifert, M.H., Jakob, P.M., Jellus, V., Haase, A. and Hillenbrand, C. (2000) High-resolution diffusion imaging using a radial turbo-spin-echo sequence: implementation, eddy current compensation, and self-navigation. J Magn Reson, 144, 243-54. (TP A1, A7)

20. Griswold, M.A., Jakob, P.M., Nittka, M., Goldfarb, J.W. and Haase, A. (2000) Partially parallel imaging with localized sensitivities (PILS). Magn Reson Med, 44, 602-9. (TP A1, A7)

21. Waller, C., Kahler, E., Hiller, K.H., Hu, K., Nahrendorf, M., Voll, S., Haase, A., Ertl, G. and Bauer, W.R. (2000) Myocardial perfusion and intracapillary blood volume in rats at rest and with coronary dilatation: MR imaging in vivo with use of a spin-labeling technique. Radiology, 215, 189-97. (TP A1, A6, A8, B9)

22. Nahrendorf, M., Wiesmann, F., Hiller, K.H., Han, H., Hu, K., Waller, C., Ruff, J., Haase, A., Ertl, G. and Bauer, W.R. (2000) In vivo assessment of cardiac remodeling after myocardial infarction in rats by cine.magnetic resonance imaging. J Cardiovasc Magn Reson, 2, 171-80. (TP A1, A6, A8, B9)

23. Eberli, F.R., Stromer, H., Ferrell, M.A., Varma, N., Morgan, J.P., Neubauer, S. and Apstein, C.S. (2000) Lack of direct role for calcium in ischemic diastolic dysfunction in isolated hearts. Circulation, 102, 2643-9. (TP A3)

24. Neubauer, S. (2000a) Cardiac magnetic resonance spectroscopy: potential clinical applications. Herz, 25, 452-60. (TP A3)

25. Neubauer, S. (2000b) Clinical relevance of MR spectroscopy of the heart. Z Kardiol, 89, 74-7. (TP A3)

26. Saupe, K.W., Spindler, M., Hopkins, J.C., Shen, W. and Ingwall, J.S. (2000) Kinetic, thermodynamic, and developmental consequences of deleting creatine kinase isoenzymes from the heart. Reaction kinetics of the creatine kinase isoenzymes in the intact heart. J Biol Chem, 275, 19742-6. (TP A3)

27. Stromer, H., de Groot, M.C., Horn, M., Faul, C., Leupold, A., Morgan, J.P., Scholz, W. and Neubauer, S. (2000) Na(+)/H(+) exchange inhibition with HOE642 improves postischemic recovery due to attenuation of Ca(2+) overload and prolonged acidosis on reperfusion. Circulation, 101, 2749-55. (TP A3)

28. Schneider, J., Fekete, E., Weisser, A., Neubauer, S. and von Kienlin, M. (2000) Reduced (1)H-NMR visibility of creatine in isolated rat hearts. Magn Reson Med, 43, 497-502. (TP A3, A7)

29. Hammerschmidt, S., Bell, M., Buchler, N., Wahn, H., Remkes, H., Lohse, M.J. and Neubauer, S. (2000) Acute changes of myocardial creatine kinase gene expression under beta-adrenergic stimulation. Biochim Biophys Acta, 1502, 471-80. (TP A3, C9)

30. Taylor, A.M., Thorne, S.A., Rubens, M.B., Jhooti, P., Keegan, J., Gatehouse, P.D., Wiesmann, F., Grothues, F., Somerville, J. and Pennell, D.J. (2000) Coronary artery imaging in grown up congenital heart disease: complementary role of magnetic resonance and x-ray coronary angiography. Circulation, 101, 1670-8. (TP A6)

31. Gaudron, P., Kugler, l., Hu, K., Fraccarollo, D., Bauer, W., Eilles, C. and Ertl, G. (2000) Effect of quinapril initiated during progressive remodeling in asymptomatic patients with healed myocardial infarction. Am J Cardiol, 86, 139-44. (TP A8, B9)

32. Sauzeau, V., Le Jeune, H., Cario-Toumaniantz, C., Smolenski, A., Lohmann, S.M., Bertoglio, J., Chardin, P., Pacaud, P. and Loirand, G. (2000) Cyclic GMP-dependent protein kinase signaling pathway inhibits RhoA-induced Ca2+ sensitization of contraction in vascular smooth muscle. J Biol Chem, 275, 21722-9. (TP B4)

33. Butt, E., Bernhardt, M., Smolenski, A., Kotsonis, P., Frohlich, L.G., Sickmann, A., Meyer, H.E., Lohmann, S.M. and Schmidt, H.H. (2000) Endothelial nitric-oxide synthase (Type III) is activated and becomes calcium independent upon phosphorylation by cyclic nucleotide-dependent protein kinases. J Biol Chem, 275, 5179-87. (TP B4, C7)

34. Oelze, M., Mollnau, H., Hoffmann, N., Warnholtz, A., Bodenschatz, M., Smolenski, A., Walter, U., Skatchkov, M., Meinertz, T. and Munzel, T. (2000) Vasodilator-stimulated phosphoprotein serine 239 phosphorylation as a sensitive monitor of defective nitric Oxide/cGMP signaling and endothelial dysfunction. Circ Res, 87, 999-1005. (TP B4, C8)

35. Schwarz, U.R., Kobsar, A.L., Koksch, M., Walter, U. and Eigenthaler, M. (2000) Inhibition of agonist-induced p42 and p38 mitogen-activated protein kinase phosphorylation and CD40 ligand/P-selectin expression by cyclic nucleotide-regulated pathways in human platelets. Biochem Pharmacol, 60, 1399-407. (TP B4, C8)

36. Smolenski, A., Poller, W., Walter, U. and Lohmann, S.M. (2000) Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I. J Biol Chem, 275, 25723-32. (TP B4, C8)

37. Pelzer, T., Schumann, M., Neumann, M., deJager, T., Stimpel, M., Serfling, E. and Neyses, L. (2000) 17beta-estradiol prevents programmed cell death in cardiac myocytes. Biochem Biophys Res Commun, 268, 192-200. (TP B6)

38. Witte, K., Hu, K., Swiatek, J., Mussig, C., Ertl, G. and Lemmer, B. (2000) Experimental heart failure in rats: effects on cardiovascular circadian rhythms and on myocardial beta-adrenergic signaling. Cardiovasc Res, 47, 350-8. (TP B9)

39. Bauersachs, J., Braun, C., Fraccarollo, D., Widder, J., Ertl, G., Schilling, L., Kirchengast, M. and Rohmeiss, P. (2000a) Improvement of renal dysfunction in rats with chronic heart failure after myocardial infarction by treatment with the endothelin A receptor antagonist, LU 135252. J Hypertens, 18, 1507-14. (TP B9, B10)

40. Bauersachs, J., Fraccarollo, D., Ertl, G., Gretz, N., Wehling, M. and Christ, M. (2000b) Striking increase of natriuresis by low-dose spironolactone in congestive heart failure only in combination with ACE inhibition : mechanistic evidence to support RALES. Circulation, 102, 2325-8. (TP B9, B10)

41. Bauersachs, J., Fraccarollo, D., Galuppo, P., Widder, J. and Ertl, G. (2000c) Endothelin-receptor blockade improves endothelial vasomotor dysfunction in heart failure. Cardiovasc Res, 47, 142-9. (TP B9, B10)

42. Widder, J., Bauersachs, J., Fraccarollo, D., Ertl, G. and Schilling, L. (2000) Endothelium-dependent and -independent vasoreactivity of rat basilar artery in chronic heart failure. J Cardiovasc Pharmacol, 35, 515-22. (TP B9, B10)

43. Ball L.J., Kuhne, R., Hoffmann, B., Hafner, A., Schmieder, P., Volkmer-Engert, R., Hof, M., Wahl, M., Schneider-Mergener, J., Walter, U., Oschkinat, H. and Jarchau, T. (2000) Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity. Embo J, 19, 4903-14. (TP C3, C8)

44. Golenhofen, N. and Drenckhahn, D. (2000) The catenin, p120ctn, is a common membrane-associated protein in various epithelial and non-epithelial cells and tissues. Histochem Cell Biol, 114, 147-55. (TP C6)

45. Komarov, A.M., Wink, D.A., Feelisch, M. and Schmidt, H.H. (2000) Electron-paramagnetic resonance spectroscopy using N-methyl-D-glucamine dithiocarbamate iron cannot discriminate between nitric oxide and nitroxyl: implications for the detection of reaction products for nitric oxide synthase. Free Radic Biol Med, 28, 739-42. (TP C7)

46. Kotsonis, P., Frohlich, L.G., Shutenko, Z.V., Horejsi, R., Pfleiderer, W. and Schmidt, H.H. (2000) Allosteric regulation of neuronal nitric oxide synthase by tetrahydrobiopterin and suppression of auto-damaging superoxide. Biochem J, 346 Pt 3, 767-76. (TP C7)

47. Becker, E.M., Schmidt, P., Schramm, M., Schroder, H., Walter, U., Hoenicka, M., Gerzer, R. and Stasch, J.P. (2000) The vasodilator-stimulated phosphoprotein (VASP): target of YC-1 and nitric oxide effects in human and rat platelets. J Cardiovasc Pharmacol, 35, 390-7. (TP C8)

48. Engelhardt, S. and Lohse, M.J. (2000) Determination of adrenergic receptor mRNAs by quantitative reverse transcriptase-polymerase chain reactions. Methods Mol Biol, 126, 155-68. (TP C9)

49. Jahns, R., Boivin, V., Krapf, T., Wallukat, G., Boege, F. and Lohse, M.J. (2000) Modulation of beta1-adrenoceptor activity by domain-specific antibodies and heart failure-associated autoantibodies. J Am Coll Cardiol, 36, 1280-7. (TP C9)

50. Ruiz-Gomez, A., Humrich, J., Murga, C., Quitterer, U., Lohse, M.J. and Mayor, F., Jr. (2000) Phosphorylation of phosducin and phosducin-like protein by G protein-coupled receptor kinase 2. J Biol Chem, 275, 29724-30. (TP C9)

SFB 355 Publications 2001: Articles

(A1-C10. projects involved)

1. Sandstede, J.J., Pabst, T., Wacker, C., Wiesmann, F., Hoffmann, V., Beer, M., Kenn, W., Bauer, W. and Hahn, D. (2001) Breath-hold 3D MR coronary angiography with a new intravascular contrast agent (feruglose)-first clinical experiences. Magn Reson Imaging, 19, 201-5. (TP A1, A3, A6, A8)

2. Sandstede, J.J., Pabst, T., Beer, M., Lipke, C., Baurle, K., Butter, F., Harre, K., Kenn, W., Voelker, W., Neubauer, S. and Hahn, D. (2001a) Assessment of myocardial infarction in humans with (23)Na MR imaging: comparison with cine MR imaging and delayed contrast enhancement. Radiology, 221, 222-8. (TP A1, A3)

3. Nahrendorf, M., Wiesmann, F., Hiller, K.H., Hu, K., Waller, C., Ruff, J., Lanz, T.E., Neubauer, S., Haase, A., Ertl, G. and Bauer, W.R. (2001) Serial cine-magnetic resonance imaging of left ventricular remodeling after myocardial infarction in rats. J Magn Reson Imaging, 14, 547-55. (TP A1, A3, A6, A8, B9)

4. Pabst, T., Sandstede, J., Beer, M., Kenn, W., Greiser, A., von Kienlin, M., Neubauer, S. and Hahn, D. (2001) Optimization of ECG-triggered 3D (23)Na MRI of the human heart. Magn Reson Med, 45, 164-166. (TP A1, A3, A7)

5. Wiesmann, F., Ruff, J., Engelhardt, S., Hein, L., Dienesch, C., Leupold, A., Illinger, R., Frydrychowicz, A., Hiller, K.H., Rommel, E., Haase, A., Lohse, M.J. and Neubauer, S. (2001) Dobutamine-stress magnetic resonance microimaging in mice : acute changes of cardiac geometry and function in normal and failing murine hearts. Circ Res, 88, 563-9. (TP A1, A3, A6, C9, C10)

6. Beer, M., Buchner, S., Sandstede, J., Viehrig, M., Lipke, C., Krug, A., Kostler, H., Pabst, T., Kenn, W., Landschutz, W., von Kienlin, M., Harre, K., Neubauer, S. and Hahn, D. (2001) 31P-MR Spectroscopy for the evaluation of energy metabolism in intact residual myocardium after acute myocardial infarction in humans. Magma, 13, 70-5. (TP A1, A3, A7)

7. von Kienlin, M., Beer, M., Greiser, A., Hahn, D., Harre, K., Kostler, H., Landschutz, W., Pabst, T., Sandstede, J. and Neubauer, S. (2001) Advances in human cardiac 31P-MR spectroscopy: SLOOP and clinical applications. J Magn Reson Imaging, 13, 521-7. (TP A1, A3, A7)

8. Beer, M., Sandstede, J., Weilbach, F., Spindler, M., Buchner, S., Krug, A., Kostler, H., Pabst, T., Kenn, W., Landschutz, W., von Kienlin, M., Toyka, K.V., Ertl, G., Gold, R. and Hahn, D. (2001b) Cardiac metabolism and function in patients with multiple sclerosis: a combined 31P-MR-spectroscopy and MRI study. Rofo Fortschr Geb Rontgenstr Neuen Bildgeb Verfahr, 173, 399- 404. (TP A1, A3, A7, B9)

9. Bauer, W.R., Hiller, K.H., Galuppo, P., Neubauer, S., Kopke, J., Haase, A., Waller, C. and Ertl, G. (2001) Fast High-Resolution Magnetic Resonance Imaging Demonstrates Fractility of Myocardial Perfusion in Microscopic Dimensions. Circ Res, 88, 340-346. (TP A1, A6, A8, B9)

10. Hiller, K.H., Waller, C., Voll, S., Haase, A., Ertl, G. and Bauer, W.R. (2001) Combined high-speed NMR imaging of perfusion and microscopic coronary conductance vessels in the isolated rat heart. Microvasc Res, 62, 327-34. (TP A1, A6, A8, B9)

11. Waller, C., Hiller, K.H., Kahler, E., Hu, K., Nahrendorf, M., Voll, S., Haase, A., Ertl, G. and Bauer, W.R. (2001) Serial magnetic resonance imaging of microvascular remodeling in the infarcted rat heart. Circulation, 103, 1564-9. (TP A1, A6, A8, B9)

12. Waller, C., Hiller, K.H., Voll, S., Haase, A., Ertl, G. and Bauer, W.R. (2001b) Myocardial perfusion imaging using a non-contrast agent MR imaging technique. Int J Card Imaging, 17, 123-32. (TP A1, A6, A8, B9)

13. Wiesmann, F., Beer, M., Krause, U., Pabst, T., Kenn, W., Hahn, D. and Ertl, G. (2001a) Clubbing due to peripheral hypervascularization: recognition by contrast-enhanced, three-dimensional magnetic resonance angiography. Circulation, 104, 2503.
(TP A1, A6, B9)

14. Brede, M., Hadamek, K., Meinel, L., Wiesmann, F., Peters, J., Engelhardt, S., Simm, A., Haase, A., Lohse, M.J. and Hein, L. (2001) Vascular hypertrophy and increased P70S6 kinase in mice lacking the angiotensin II AT(2) receptor. Circulation, 104, 2602-7. (TP A1, A6, C9, C10)

15. Horn, M., Weidensteiner, C., Scheffer, H., Meininger, M., de Groot, M., Remkes, H., Dienesch, C., Przyklenk, K., von Kienlin, M. and Neubauer, S. (2001) Detection of myocardial viability based on measurement of sodium content: A (23)Na-NMR study. Magn Reson Med, 45, 756-64. (TP A3, A7)

16. Spindler, M., Burrows, G., Kowallik, P., Ertl, G. and Voelker, W. (2001a) Postpericardiotomy syndrome and cardiac tamponade as a late complication after pacemaker implantation. Pacing Clin Electrophysiol, 24, 1433-4. (TP A3, B9)

17. Spindler, M., Illing, B., Horn, M., de Groot, M., Ertl, G. and Neubauer, S. (2001b) Temporal fluctuations of myocardia high-energy phosphate metabolite with the cardiac cycle. Basic Res Cardiol, 96, 553-6. (TP A3, B9)

18. Gaudron, P., Kugler, I., Hu, K., Bauer, W., Eilles, C. and Ertl, G. (2001) Time course of cardiac structural, functional and electrical changes in asymptomatic patients after myocardial infarction: their inter-relation and prognostic impact. J Am Coll Cardiol, 38, 33-40. (TP A8, B9)

19. de Vente, J., Asan, E., Gambaryan, S., Markerink-van Ittersum, M., Axer, H., Gallatz, K., Lohmann, S.M. and Palkovits, M. (2001) Localization of cGMP-dependent protein kinase type II in rat brain. Neuroscience, 108, 27-49. (TP B4)

20. Fischer, T.A., Ludwig, S., Flory, E., Gambaryan, S., Singh, K., Finn, P., Pfeffer, M.A., Kelly, R.A. and Pfeffer, J.M. (2001) Activation of cardiac c-Jun NH(2)-terminal kinases and p38-mitogen-activated protein kinases with abrupt changes in hemodynamic load. Hypertension, 37, 1222-8. (TP B4)

21. Hanada, S., Terada, Y., Inoshita, S., Sasaki, S., Lohmann, S.M., Smolenski, A. and Marumo, F. (2001) Overexpression of protein kinase G using adenovirus inhibits cyclin E transcription and mesangial cell cycle. Am J Physiol Renal Physiol, 280, F851-9. (TP B4)

22. Arancio, O., Antonova, I., Gambaryan, S., Lohmann, S.M., Wood, J.S., Lawrence,
D.S. and Hawkins, R.D. (2001) Presynaptic role of cGMP-dependent protein kinase during long-lasting potentiation. J Neurosci, 21, 143-9. (TP B4)

23. Pierkes, M., Gambaryan, S., Boknik, P., Lohmann, S.M., Schmitz, W., Potthast, R., Holtwick, R. and Kuhn, M. (2001) Increased effects of C-type natriuretic peptide on cardiac ventricular contractility and relaxation in guanylyl cyclase A-deficient mice. Cardiovascular Research, in press. (TP B4)

24. Mulsch, A., Oelze, M., Kloss, S., Mollnau, H., Topfer, A., Smolenski, A., Walter, U., Stasch, J.P., Warnholtz, A., Hink, U., Meinertz, T. and Munzel, T. (2001) Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Stimulated Phosphoprotein in Aorta. Circulation, 103, 2188-94. (TP B4, C8)

25. Fischer, T.A., Palmetshofer, A., Gambaryan, S., Butt, E., Jassoy, C., Walter, U., Sopper, S. and Lohmann, S.M. (2001b) Activation of cGMP-dependent protein kinase Ibeta inhibits interleukin 2 release and proliferation of T cell receptor-stimulated human peripheral T cells. J Biol Chem, 276, 5967-74. (TP B4, C8)

26. De Jager, T., Pelzer, T., Mueller-Botz, S., Imam, A., Muck, J. and Neyses, L. (2001) Mechanisms of estrogen receptor action in the myocardium: rapid gene activation via the ERK1/2 pathway and serum response elements. J Biol Chem, 2, 2. (TP B6)

27. Pelzer, T., Neumann, M., de Jager, T., Jazbutyte, V. and Neyses, L. (2001) Estrogen effects in the myocardium: inhibition of nf-kappab dna binding by estrogen receptor-alpha and -beta. Biochem Biophys Res Commun, 286, 1153-7. (TP B6)

28. Piuhola, J., Hammes, A., Schuh, K., Neyses, L., Vuolteenaho, O. and Ruskoaho, H. (2001) Overexpression of sarcolemmal calcium pump attenuates induction of cardiac gene expression in response to ET-1. Am J Physiol Regul Integr Comp Physiol, 281, R699-705. (TP B6)

29. Schuh, K., Uldrijan, S., Telkamp, M., Rothlein, N. and Neyses, L. (2001) The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I. J Cell Biol, 155, 201-6. (TP B6)

30. Hu, K., Bahner, U., Gaudron, P., Palkovits, M., Ring, M., Fehle, A., Kruse, B. and Ertl, G. (2001) Chronic effects of ACE-inhibition (quinapril) and angiotensin-II-type-1 receptor blockade (losartan) on atrial natriuretic peptide in brain nuclei of rats with experimental myocardial infarction. Basic Res Cardiol, 96, 258-66. (TP B9)

31. Staufenberger, S., Jacobs, M., Brandstatter, K., Hafner, M., Regitz-Zagrosek, V., Ertl, G. and Schorb, W. (2001) Angiotensin II type 1 receptor regulation and differential trophic effects on rat cardiac myofibroblasts after acute myocardial infarction. J Cell Physiol, 187, 326-35. (TP B9)

32. Bauersachs, J., Fleming, I., Fraccarollo, D., Busse, R. and Ertl, G. (2001a) Prevention of endothelial dysfunction in heart failure by vitamin E. Attenuation of vascular superoxide anion formation and increase in soluble guanylyl cyclase expression. Cardiovasc Res, 51, 344-50. (TP B9, B10)

33. Bauersachs, J., Galuppo, P., Fraccarollo, D., Christ, M. and Ertl, G. (2001b) Improvement of left ventricular remodeling and function by hydroxymethylglutaryl coenzyme a reductase inhibition with cerivastatin in rats with heart failure after myocardial infarction. Circulation, 104, 982-5. (TP B9, B10)

34. Wagner, J.A., Hu, K., Bauersachs, J., Karcher, J., Wiesler, M., Goparaju, S.K., Kunos, G. and Ertl, G. (2001) Endogenous cannabinoids mediate hypotension after experimental myocardial infarction. J Am Coll Cardiol, 38, 2048-2054. (TP B9, B10, B12)

35. Maier, S., Lange, V., Simm, A., Walter, U. and Kirstein, M. (2001) Insulin fails to modulate the cardiac L-type Ca2+ current in Type II diabetes patients--a possible link to cardiac dysfunction in diabetes mellitus. Diabetologia, 44, 269. (TP C8)

36. Marienfeld, U., Walter, U. and Simm, A. (2001) Inhibition of rat cardiac fibroblast growth by cAMP--but not by cGMP-dependent protein kinase. Basic Res Cardiol, 96, 184-91. (TP C8)

37. Boivin, V., Jahns, R., Gambaryan, S., Ness, W., Boege, F. and Lohse, M.J. (2001) Immunofluorescent imaging of beta1- and beta2-adrenergic receptors in rat kidney. Kidney Int, 59, 515-531. (TP B4, C9)

38. Krasel, C., Dammeier, S., Winstel, R., Brockmann, J., Mischak, H. and Lohse, M.J. (2001) Phosphorylation of GRK2 by Protein Kinase C Abolishes Its Inhibition by Calmodulin. J Biol Chem, 276, 1911-1915. (TP C9)

39. Lohse, M.J. and Engelhardt, S. (2001) Protein kinase a transgenes: the many faces of cAMP. Circ Res, 89, 938-40. (TP C9)

40. Kaumann, A.J., Engelhardt, S., Hein, L., Molenaar, P. and Lohse, M. (2001) Abolition of (-)-CGP 12177-evoked cardiostimulation in double beta1/beta2-adrenoceptor knockout mice. Obligatory role of beta1- adrenoceptors for putative beta4-adrenoceptor pharmacology. Naunyn Schmiedebergs Arch Pharmacol, 363, 87-93. (TP C9)

41. Engelhardt, S., Grimmer, Y., Fan, G.H. and Lohse, M.J. (2001b) Constitutive activity of the human beta(1)-adrenergic receptor in beta(1)-receptor transgenic mice. Mol Pharmacol, 60, 712-7. (TP C9)

42. Chruscinski, A., Brede, M.E., Meinel, L., Lohse, M.J., Kobilka, B.K. and Hein, L. (2001) Differential Distribution of beta-Adrenergic Receptor Subtypes in Blood Vessels of Knockout Mice Lacking beta(1)- or beta(2)-Adrenergic Receptors. Mol Pharmacol, 60, 955-62. (TP C9, C10)

43. Engelhardt, S., Boknik, P., Keller, U., Neumann, J., Lohse, M.J. and Hein, L. (2001) Early impairment of calcium handling and altered expression of junctin in hearts of mice overexpressing the b1-adrenergic receptor. Faseb J, 15, 15. (TP C9, C10)

44. Hein, L. (2001c) Transgenic models of alpha 2-adrenergic receptor subtype function. Rev Physiol Biochem Pharmacol, 142, 161-85. (TP C10)

University Hospital, Würzburg