Studies on the mechansms of inflammation induced by polymicrobial infections employing the adult periodontitis model
Project management at the University of Würzburg:
Aggressive periodontitis is caused by deregulated inflammation triggered by a polymicrobial infection of the gingiva. The course of the disease is determined by genetic predisposition and by the virulence of distinct pathogens. Previous investigations both in vitro and in vivo contributed to our understanding of the disease. However, neither the polymicrobial genesis of the disease nor the importance of non-cultivable organisms have been considered appropriately until now. Therefore, in this application in vitro cultured or patient derived periodontal bacteria will be investigated for their interaction with the humoral immune system as biofilms or as planctonic cells. By using species-specific 16S rDNA probes the target cells of complement factors and antibodies will be determined. The methods to be used are flow cytometry and confocal scanning laser microscopy (CSLM). The project will elucidate the role of biofilm formation for the modulation of inflammation. It will provide insight into the question whether non-cultivable organisms play a role as triggers of inflammation.
Projekt period: from 05.2002 to 04.2004