Culturing and characterization of normal colon epithelial, adenoma and carcinoma cells to establish an in vitro adenoma carcinoma cell culture model
Project management at the University of Würzburg:
The adenoma carcinoma sequence is a well accepted model of colon cancer car-cinogenesis. The stepwise development of colon cancer is accompanied by distinct genetic changes modulated by exogenic factors. These factors may be important for primary prevention of colon cancer. In vitro testing of these substances should be performed using normal human epithelial cell cultures. Because of the lack of well characterized long term cultures of colonic epithelial cells, in most studies colon carcinoma cell lines are employed. Results of these studies may be misleading. The aim of this study is the establishment of long term human colon epithelial cell cultures. Firstly short term culture is to be optimized. These cell will be immortilized using telomerase reverse transcriptase. Culturing of adeno-ma and carcinoma cell lines is already well established and is performed simultanously. All cultivated cell lines will be characterized by molecular (cDNA array, allelotyping) and cyto-genetic methods (SKY, CGH). Long term cuturing of normal epithelial cell, adenoma and carcinoma cells as a model of colon cancer carcinogenesis will facilitate reliable studies of colon cancer prevention by exogenous factors.
Projekt period: from 05.2002 to 04.2004